NIV - Dr. Shailesh D Pawar

Dr. Shailesh D Pawar

Designation: Scientist-F & Officer-In-Charge

Division:ICMR-National Institute of Virology, Mumbai Unit (Formerly Enterovirus Research Centre), Poliovirus Essential Facility.

Educational qualifications:M.Sc., Ph. D (Microbiology)

Date of joining ICMR-NIV: August 1995

Contact: 020-26006848
Email: pawar.sd@gov.in
Office: Indian Council of Medical Research-National Institute of Virology Pune Maharashtra - 411001

Research Interests:

Polio
Measles
Rubella
Influenza
Containment of Polioviruses
Laboratory Quality Management

Awards/Honours:

Indian Council of Medical Research Young Scientist Award-2010 (Shakuntala Amir Chand Award) for the significant contribution in Biomedical Research from the ICMR, Govt. of India, New Delhi.
Pune Municipal Corporations’ Young Scientist Award-2015, for notable contribution for Science in the area of Epidemiology & Public Health.
Rajarshee Shahu Maharaj Social Foundation Award-2020, for the outstanding contribution for the Society in the area of medical research and public health, 26 June 2020.
Poster Presentation Award (First Prize) on “Jal Sankat and Jal Sarakshan” in Hindi Workshop, held at the ICMR-NIV, Pune 20-9-2019.
Scholarship for distinction in S.S.C. Board examination.
First rank in B.Sc. Microbiology, Ahmednagar College, Pune University.
Letter of honor from Rotary Club, Pune, for significant contribution for the Society, 2015
Reviewer Award, Journal of Virological Methods, 2015
First rank in B.Sc. Microbiology, Ahmednagar College, Pune University, 1993.
Third prize in school debate competition, October 22, 1986.
Member: Global Polio Laboratory Network, World Health Organization.
Member of the Expert BSL-3 Audit Group of ICMR, for certification of BSL-3 laboratories in India.
DBT Nominee, for the Institutional Biosafety Committees, Government of India.
Member of Asia Pacific Congress of Medical Virology.
Member of Society for Biosafety, India.

ORCID ID:0000-0002-0222-8055

Researcher ID:ABB-1031-2021

Google Scholar Profile Link

List of Publications

1. Hong Sun, Chelsea Harrington, Nancy Gerloff, Mark Mandelbaum, Stacey Jeffries-Miles, Sharma DK, Pawar SD et al., Validation of a redesigned pan-poliovirus assay and real-time PCR platforms for the global poliovirus laboratory network, PLOS One, 2021. 6, 16, e0255795. doi: 10.1371/journal.pone.0255795.
2. Pawar SD*, Tandale, B. V.; Tare, D. S.; Keng, S. S.; Kode, S. S.; Abraham, P. Classical and Molecular Virology in the Context of SARS-CoV-2, JIMA, 119, 2021, 24-30.
3. Kode SS, Pawar SD*, Tare DS, Mullick J. Application of frozen and stored glutaraldehyde-fixed turkey red blood cells for hemagglutination and hemagglutination inhibition assays for the detection and identification of influenza viruses. J Virol Methods. 2020. 14;289: 114046.
4. Pawar SD*, Kode SS, Keng SS, Tare DS, Abraham P. Steps, implementation and importance of quality management in diagnostic laboratories with special emphasis on coronavirus disease-2019. IJMM, 2020, 38, 243-251.
5. Potdar V, Vipat V, Ramdasi A, Jadhav S, Pawar-Patil J, Walimbe A, Patil SS, Choudhury ML, Shastri J, Agrawal S, Pawar SD et al., Phylogenetic classification of the whole-genome sequences of SARS-CoV-2 from India & evolutionary trends. IJMR. 2021, 153:166-174.
6. Tikute SS, Pawar SD, Kawale SS, Sharma DK, Gopalkrishna VA. A real-time polymerase chain reaction-based approach for qualitative estimation of viral RNA in organ tissues of coxsackievirus A-16-infected neonatal mice. IJMM, 2020, 38, 464-468.
7. Sapkal G, et al., COVID Support Team includes Pawar SD. Development of indigenous IgG ELISA for the detection of anti-SARS-CoV-2 IgG., IJMR 2020, 151, 444-449.
8. Godbole NM, Sinha RA, Tiwari S, Pawar SD*, Dhole TN. Analysis of influenza virus-induced perturbation in autophagic flux and its modulation during Vitamin D3 mediated anti-apoptotic signaling. Virus Res. 282, 2020, 282:197936. doi: 10.1016/j.virusres.2020.197936.
9. Saxena VK*, Pawar SD*, Qureshi THIH, Surve P, Yadav P, Nabi F et al., Isolation and molecular characterization of coxsackievirus A6 and coxsackievirus A16 from a case of recurrent Hand, Foot and Mouth Disease in Mumbai, Maharashtra, India, 2018. Virus disease. 2020, 31, 56-60.
10. Kode SS, Pawar SD*, Tare DS, Keng SS, Mullick J. Amantadine resistance markers among low pathogenic avian influenza H9N2 viruses isolated from poultry in India, during 2009-2017. Microbial Pathogenesis, 2019, 137:103779. doi: 10.1016/j.micpath.2019.103779.
11. Kode SS, Pawar SD*, Tare DS, Keng SS, Hurt AC, Mullick J. A novel I117T substitution in neuraminidase of highly pathogenic avian influenza H5N1 virus conferring reduced susceptibility to oseltamivir and zanamivir. Vet Microbiology, 2019, 235, 21-24. doi: 10.1016/j.vetmic.2019.06.005.
12. Kode SS, Pawar SD*, Cherian SS, Tare DS, Bhoye D, Keng SS, et al., Selection of avian influenza A (H9N2) virus with reduced susceptibility to neuraminidase inhibitors oseltamivir and zanamivir. Virus Research, 2019, 265, 122-126. doi: 10.1016/j.virusres.2019.03.019.
13. Kushwaha AS, Kotwal A, Biradar CI, Mahen A, Kumar M, Pawar SD et al., Seroprevalence of influenza A H1N1 (swine) infection in the human population in a cantonment, Med J Armed Forces India, 2019, 75, 312-317. doi: 10.1016/j.mjafi.2018.08.003.
14. Dharmapalan D, Saxena VK, Pawar SD, Qureshi THH, Surve P. Clinical and Molecular Investigations of Hand Foot and Mouth Disease Outbreak in Navi Mumbai, India. Indian Pediatrics, 2019, 15, 56, 1052-1054
15. Pawar SD*, Keng SS, Tare DS, Thormothe AL, Sapkal GN, Anukumar B, et al., A virus precipitation method for the concentration and detection of avian influenza viruses from environmental water resources and its possible application in outbreak investigations. IJMR, 2019, 150, 612-619. doi: 10.4103/ijmr.IJMR_1697_18.
16. Pawar SS, Pawar YS, Kadam M, Pawar MS, Pawar SD*. Breeding of Red-Vented Bulbul Pycnonotus cafer and nest predation by Asian Koel Eudynamys scolopaceus, Ela Journal of Forestry and Wildlife, 2019, 3, 591-593.
17. Tare DS, Kode SS, Hurt AC, Pawar SD*, Assessing the susceptibility of highly pathogenic avian influenza H5N1 viruses to oseltamivir using embryonated chicken eggs. IJMR, 2019, 150, 486-491. doi: 10.4103/ijmr.IJMR_845_18.
18. Thube M, Shil P, Kasbe R, Patil AA, Pawar SD, Mullick J. Differences in Type-I interferon response in human lung epithelial cells infected by highly pathogenic H5N1 and low pathogenic H11N1 avian influenza viruses. Virus Genes, 2018, 54, 414-423. doi: 10.1007/s11262-018-1556-1.
19. Pawar SD*, Pande SA, Tare DS, Keng SS, Kode SS, Singh DK et al., Morphological and biochemical characteristics of avian faecal droppings and their impact on survival of avian influenza virus. Food Environ Virol. 2018, 10, 99-106. doi: 10.1007/s12560-017-9323-3.
20. Rattan A, Pawar SD, Nawadkar R, Kulkarni N, Lal G, Mullick J, et al., Synergy between the classical and alternative pathways of complement is essential for conferring effective protection against the pandemic influenza A(H1N1) 2009 virus infection. PLOS Pathogens, 2017, doi.org/10.1371/journal.ppat.1006248.
21. Nehul S, Kulkarni A, Pawar SD, Godbole S, Ghate M, Thakar M. Cross-reactive influenza-specific antibody-dependent cellular cytotoxicity-mediating antibodies in HIV-infected Indian individuals. Infectious Diseases, 2018, 50, 35-43. doi: 10.1080/23744235.2017.1361547.
22. Gurav YK*, Chadha MS, Tandale BV, Potdar VA, Pawar SD, Shil P et al., Influenza A(H1N1)pdm09 outbreak detected in inter-seasonal months during the surveillance of influenza-like illness in Pune, India, 2012-2015. Epidemiol. Infect., 2017, doi:10.1017/S0950268817000553.
23. Yadav PD, Sudeep AB, Pawar SD, Shete A, Patil D, et al., Circulation of Nipah virus in Pteropus giganteus bats, Northeast region of India, 2015. IJMR, 2018, 147, 318-320. doi: 10.4103/ijmr.IJMR_1488_16.
24. Pawar SD, Tandale BV, Mali RS, Potdar VA, Kode SS, Biswas D, et al., Avian influenza A H7N9 virus infections not evident among high-risk groups in India, 2014. IJMR, 2016, 144, 942-944. doi: 10.4103/ijmr.IJMR_718_15.
25. Pawar SD*, Jamgaonkar AV, Umarani UB, Kode SS. Seroepidemiology of avian influenza H5N1, H9N2, and Newcastle disease viruses during 1954 to 1981 in India. IJMR, 2016, 144, 472-476. doi: 10.4103/0971-5916.198666.
26. Eichelberger MC et al., ELLA study participants which include Pawar SD, Kode SS, Wood J. Comparability of neuraminidase inhibition antibody titers measured by enzyme-linked lectin assay (ELLA) for the analysis of influenza vaccine immunogenicity. Vaccine. 2016, 20, 34, 458-65. doi: 10.1016/j.vaccine.2015.12.022.
27. Damle RG, Patil AA, Bhide VS, Pawar SD, Sapkal GN, Bondre VP. Development of a novel rapid micro-neutralization ELISA for the detection of neutralizing antibodies against Chandipura virus. J Virol Methods. 2016, 240:1-6. doi: 10.1016/j.jviromet.2016.11.007.
28. Yadav P, Sarkale P, Patil D, Shete A, Kokate P, Kumar V, Jain R, Jadhav S, Basu A, Pawar SD et al., Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India. Infection, Genetics & Evolution, 2016, 45, 224-229. doi: 10.1016/j.meegid.2016.09.010.
29. Pawar SD*, Murtadak VB, Kale SD, Shinde PV, Parkhi SS. Evaluation of different inactivation methods for high and low pathogenic avian influenza viruses in egg-fluids for antigen preparation. J Virol Methods, 2015, 15, 222, 28-33. doi: 10.1016/j.jviromet.2015.05.004.
30. Tare DS, Pawar SD*, Use of embryonated chicken egg as a model to study the susceptibility of avian influenza H9N2 viruses to oseltamivir carboxylate, 2015, J Virol Methods, 2015, 224, 67-72. doi: 10.1016/j.jviromet.2015.08.009.
31. Laurie KL, Engelhardt OG, Wood J, Heath A, Katz JM, Peiris M, Hoschler K, Hungnes O, Zhang W, Van Kerkhove MD; CONSISE Laboratory Working Group participants, which includes Pawar SD. An international laboratory comparison of influenza microneutralisation assay protocols for A(H1N1)pdm09, A(H3N2) and A(H5N1) influenza A viruses by CONSISE. Clin Vaccine Immunol. 2015, 22, 957-64. doi: 10.1128/CVI.00278-15.
32. Pawar SD*, Tandale BV, Gurav YK, Parkhi YK, Kode SS. Immunity status against influenza A subtype H7N9 and other avian influenza viruses in a high-risk group and the general population in India. J Infect Dis. 2014, 210, 160-161. doi: 10.1093/infdis/jiu033.
33. Pawar SD, Parkhi SS, Koratkar SS, Mishra AC. Receptor specificity and erythrocyte binding preferences of avian influenza viruses isolated from India. Virology J, 2012, 2012, 30, 9, 251. doi: 10.1186/1743-422X-9-251.
34. Pawar SD, Kale SD, Rawankar AS, Koratkar SS, Raut CG, Pande SA, et al., Avian influenza surveillance reveals presence of low pathogenic avian influenza viruses in poultry during 2009-2011 in the West Bengal State, India. Virology J, 2012, 7, 151. doi: 10.1186/1743-422X-9-151.
35. Pawar SD Tandale BV, Raut CG, Parkhi SS, Barde TD, Gurav YK, et al., Avian Influenza H9N2 Seroprevalence among Poultry Workers in Pune, India, 2010. PLoS ONE, 2012, 7: e36374.doi:10.1371/journal.pone.0036374.
36. Pawar SD, Chakrabarti A, Cherian S, Pande S, Nanaware M, Raut S, et al., A Novel Avian Influenza A(H11N1) virus from wild Eurasian Spoonbills revealing a Eurasian-American genetic reassortment. Virus Genes, 2010, 41, 14-22. doi: 10.1007/s11262-010-0487-2.
37. Pawar, SD, Pande SA, Jamgaonkar A, Koratkar S, Pal B, Raut S et al., Avian influenza surveillance in wild migratory, resident, domestic birds and in poultry in Maharashtra and Manipur, India, during avian migratory season 2006-2007. Curr. Sci., 2009, 97, 550-554.
38. Tandale BV, Pawar SD, Mishra AC. Immunogenicity and safety of pandemic H1N1 2009 vaccine among adults in field use, India. Vaccine. 2011, 30, 2043-4. doi: 10.1016/j.vaccine.2011.10.008.
39. Tandale BV, Pawar SD Gurav YK, Parkhi SS, Mishra AC. Antibody persistence after Pandemic H1N1 2009 influenza vaccination among healthcare workers in Pune, India". Human Vaccines & Immunotherapeutics 9(1), 2013. 9, 125-7. doi: 10.4161/hv.22421.
40. Koratkar SS, Pawar SD*, Shelke VN, Kale SD, Mishra AC. Pathogenicity of avian influenza H11N1 virus isolated from wild aquatic bird Eurasian Spoonbill (Platalea leucorodia), IJMR, 139, 2014, 782-785.
41. Pande SA, Pawar SD, Yekale R, Kanherkar M, Khatavkar S, Deshpande P et al., Recovery of two ringed and wing-tagged Black-headed Ibises Threskiornis melanocephalus at Bhigwan, District Pune, Maharashtra. Ela Journal of Forestry and Wildlife, 2015, 4, 41-42.
42. Pawar SD, Singh A, Gangodkar SV, Rao BL. Propagation of Chandipura virus in Chick embryos. Indian J of Exp Biol., 2005, 43, 930-932.
43. Khare D, Godbole NM, Pawar SD, Mohan V, Pandey G, Gupta S et al., Calcitriol [1, 25[OH]2 D3] pre- and post- treatment suppresses inflammatory response to influenza A (H1N1) infection in human lung A549 epithelial cells. Eur J Nutr. 2013, 52, 1405-15. doi: 10.1007/s00394-012-0449-7.
44. Chakrabarti AK, Pawar SD, Cherian SS, Koratkar SS, Jadhav SM, Pal B et al., Characterization of the Influenza A H5N1 viruses of the 2008 outbreaks in India reveals a third introduction and possible endemicity, PLOS One, 2009, 4, e7846. doi: 10.1371/journal.pone.0007846.
45. Kode SS, Pawar SD, Tandale BV, Parkhi SS, Barde TD, Mishra AC. Low Level of Cross-Reactive Antibodies to Pandemic Influenza (H1N1) 2009 Virus in Humans in Pre-Pandemic Period in Maharashtra, India. Ind J Virol. 2012, 23, 36-8. doi: 10.1007/s13337-011-0055-y.
46. Gurav YK, Pawar SD, Chadha MS, Potdar VA, Deshpande AS, Koratkar SS, et al., An outbreak of pandemic influenza A(H1N1) in a boarding school in Panchgani, Maharashtra, India in June-July 2009. IJMR 132, 2010, 67-71.
47. Tandale BV, Pawar SD Gurav YK, Chadha MS, Koratkar SS, Shelke VN, et al., Seroepidemiology of pandemic influenza A (H1N1) 2009 virus infections in Pune, India. BMC Infectious Diseases, 2010, 25, 10, 255. doi: 10.1186/1471-2334-10-255.
48. Kale SD, Mishra AC, Pawar SD*, Suitability of specimen types for isolation of avian influenza viruses from poultry. Indian J. Virol., 2013, 24, 391-3. doi: 10.1007/s13337-013-0146-z.
49. Shinde PV, Koratkar SS, Pawar SD, Kale SD, Rawankar AS, Mishra AC*. Serological evidence of avian influenza H9N2 and paramyxovirus type 1 infection in emus (Dromaius novaehollandiae) in India. Avian Dis. 2012, 56, 257-260. doi: 10.1637/9878-080511-Case.1.
50. Van Kerkhove MD, Hirve S, Koukounari A and Mounts AW., for the H1N1pdm serology working group, which includes Pawar SD. Estimating Age-Specific Global Infection Rates for the 2009 Influenza Pandemic: a Meta-Analysis of A(H1N1)pdm09 Serological Studies. 2013. Influenza & Other Resp. Viruses. 2013, 7, 872-86. doi:10.1111/irv.12074.
51. Sabale SS, Pawar SD*, More BK, Mishra AC et al., Seroprevalence of pandemic influenza H1N1 (2009) and seasonal influenza viruses in pigs in Maharashtra and Gujarat states, India, 2011. IJMR, 2013, 138, 267-269.
52. Mourya DT, Yadav PD, Shete AM, Gurav YK, Raut CG, Jadi RS, Pawar SD et al., Detection, Isolation and Confirmation of Crimean-Congo Hemorrhagic Fever Virus in Human, Ticks, and Animals in Ahmadabad, India, 2010-2011. PLoS Negl Trop Dis, 2012, 6, e1653. doi:10.1371/journal.pntd.0001653.
53. Chakrabarti AK, Vipat VC, Mukherjee S, Singh R, Pawar SD, Mishra AC.. Host gene expression profiling in influenza A virus-infected lung epithelial (A549) cells: a comparative analysis between highly pathogenic and modified H5N1 viruses. Virology J, 2010, 7:219. doi: 10.1186/1743-422X-7-219
54. Ray K, Potdar VA, Cherian SS, Pawar SD, Jadhav SM, Waregaonkar SR, et al., Characterization of the complete genome of influenza A (H5N1) virus isolated during the 2006 outbreak in poultry in India, Virus Gen., 2008, 36, 345-53. doi: 10.1007/s11262-007-0195-8.
55. Mishra AC, Cherian SS, Chakrabarti AK, Pawar SD, Jadhav SM, Pal B et al., A unique influenza A (H5N1) virus causing a focal poultry outbreak in 2007 in Manipur, India. Virol J. 2009, 24, 6, 26. doi: 10.1186/1743-422X-6-26.
56. Pande, S., P. Pandit, A. Ponkshe, R. Mone, S. Pawar*, A. Mishra. Behavioral and virological studies on a rescued Oriental White-backed Vulture Gyps bengalensis from western Maharashtra, India. J. Threatened Taxa, 2011, 3, 1490-1492.
57. Mukherjee S, Vipat VC, Mishra AC, Pawar SD, Chakrabarti AK. Pandemic (H1N1) 2009 influenza virus induces weaker host immune responses in vitro: a possible mechanism of high transmissibility. Virology J, 2011, 25, 8, 140. doi: 10.1186/1743-422X-8-140.
58. Arankalle VA, Prabhakar SS, Madhukar WA, Hanumaih, Pawar SD, Mishra AC. G, N, and P Gene-based Analysis of Chandipura Viruses, India. Emerg Infect Dis., 2005, 11, 123-126.doi:10.3201/eid1101.040602.
59. BL Rao, SS Gandhe, SD Pawar, VA Arankalle, SC Shah, AA Kinkier. First Detection of Human Metapneumovirus in children with acute respiratory infection in India: a preliminary report. J Clin Microbiol., 2004, 42, 5961-5962. doi: 10.1128/JCM.42.12.5961-5962.2004.
60. Yeolekar LR, Kulkarni PB, Pawar SD, Rao BL. Influenza surveillance in Pune, India: Reappearance of B/Victoria/2/87-like influenza virus strain in 2002. Curr Sci., 2004, 86, 966-968.
61. Rao BL, Basu A, Wairagkar NS, Gore MM, Arankalle VA, Thakare JP et al., Contribution acknowledged in Rao BL et al., A large outbreak of acute encephalitis with high fatality rate in children in Andhra Pradesh, India, in 2003, associated with Chandipura Virus. Lancet, 2004; 364, 869-874. doi: 10.1016/S0140-6736(04)16982-1.
62. indicates Corresponding Author

Contribution to the WHO Guidelines

“Guidelines for the safe production and quality control of poliomyelitis vaccine”, Revision of Annex 2 of WHO Technical Report Series, No. 926, 2018. Post-Expert Committee on Biological Standardization (ECBS), doc_9 Nov 2018 E, World Health Organization, 2018."

Contribution to books

1.Mullick J, Pawar SD. Avian Influenza (Book Chapter), In National Institute of Virology’s Golden to Diamond Jubilee: The Glorious Decade. Dec. 2012, Pp. 35-63."
2.AB Sudeep, SD Pawar, Basic Virological Techniques, National Institute of Virology, Technical Manual, Diamond Jubilee Publication, Editor, Dr. AC Mishra, Dec. 2012, Pp. 83-94.
3.Wild Orchids of Northern-Western Ghats, Eds. Dr. Satish Pande, Niranjan Sant, Vivek Vishwasrao, Mandar Datar, Published by Ela Foundation (2011). (ISBN 978-81-906955-4-1). Contribution acknowledged in this book..
4.Pawar SD. Avian Influenza (Book Chapter) in Basic Ornithology: Lecture notes for the Certificate Course in Basic Ornithology. Jointly conducted by MES Abasaheb Garware College Pune and Ela Foundation, Pune. Ed. Satish Pande, 225-233, 2008..
5.Guest Editor: of an International Journal ‘Deerghayu International’, vol 16-IV, issue no 64, Oct-Dec. 2000.