NGS Includes high throughput nucleic acid sequencing technologies such as illumina (Miniseq) and proton/ion personal genome sequencing (PGM) Illumina next-generation sequencing utilizes a fundamentally different approach from the classic Sanger chain-termination method. It leverages sequencing by synthesis (SBS) technology – tracking the addition of labeled nucleotides as the DNA chain is copied – in a massively parallel fashion.
Application:
1. Targeted resequencing.
2. Small whole genome sequencing
3. Amplicon Sequencing
4. Gene expression profiling
5. SNP identification
6. Meta-genomics etc
Available NGS equipments
Make | Model | Date of installation | Status | Location |
---|---|---|---|---|
Illumina | Miniseq | 2017 | Working | NIV, Pashan, Pune |
Life Technologies (Applied Biosystems) | Ion Torrent PGM sequencer (Sr No 11C031705) | 2011 | Working | NIV, Pashan, Pune |
Life Technologies (Applied Biosystems) | Ion Torrent PGM sequencer | 2011 | Working | NIV unit, Mumbai |