The enzyme-linked immunospot (ELISpot) assay is a highly sensitive immunoassay that measures the frequency of cytokine-secreting cells at the single-cell level. The ELISpot assay is carried out in a 96-well plate, and an automated ELISpot reader is used for analysis.
Applications:
1. Screening of antibody producing cells.
2. Serial check on immune reactions towards chemicals, and newly developed pharmaceuticals.
3. Control of guided vaccinations.
4. Optimization of anti-tumour activities of dendritic cells and T-lymphocytes.
5. Diagnosis and prognostic analysis of autoimmune diseases.
6. Monitoring of immune therapy.
7. Analysis of stem cell function.
Available Equipment
| Location of installation | Make | Model | Date of Installation | Status |
|---|---|---|---|---|
| Hepatitis Group, NIV/MCC Pune | Zeiss | Axioplan2 imaging HAL 100 | 2003 |
Working |
