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Dr. Mrs. Kavita S. Lole

HomeDr. Mrs. Kavita S. Lole
HomeDr. Mrs. Kavita S. Lole
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Dr. Mrs. Kavita S. Lole

Designation: Scientist G

Division: 1) Hepatitis 2)Entomology.

Educational qualifications: M.Sc., Ph.D.

Date of joining ICMR-NIV: March, 1998

  • Contact: 9890976856
  • Email: lolekavita37@yahoo.com, lole.k@gov.in
  • Office: Indian Council of Medical Research-National Institute of Virology Pune Maharashtra - 411001

 

Research Interests:

  • Development of recombinant DNA technology based diagnostic assays and vaccine candidates
  • Understanding epidemiology of HAV and HEV
  • Understanding virus-host interactions, virus pathogenesis

 

Awards/Honours:

  • UGC (Life sciences) and CSIR Junior research fellowship (1986)
  • Cambridge Nehru scholarship (1987), but could not go due to family problems
  • Senior Research Fellowship, DBT, India (1989-1994)
  • Fogarty International Fellowship (1997-98)
  • French Govt. INSERM Fellowship (2001)

 

ORCID ID:0000-0001-6131-2268

Researcher ID:

Google Scholar Profile Link

 

List of Publications
  • 1. Mohandas S, Yadav PD, Shete A, Nyayanit D, Sapkal G, Lole K, Gupta N. SARS-CoV-2 Delta Variant Pathogenesis and Host Response in Syrian Hamsters. Viruses.2021 Sep 5;13(9):1773. doi: 10.3390/v13091773. PMID: 34578354; PMCID:PMC8473140.

  • 2. Mohandas S, Yadav PD, Nyayanit D, Shete A, Sarkale P, Hundekar S, Kumar S, Lole K. Comparison of SARS-CoV-2 Variants of Concern 202012/01 (U.K. Variant) and D614G Variant Transmission by Different Routes in Syrian Hamsters. Vector Borne Zoonotic Dis. 2021 Aug;21(8):638-641. doi: 10.1089/vbz.2021.0032. Epub 2021 Jul 1. PMID: 34197265.

  • 3. Potdar V, Vipat V, Ramdasi A, Jadhav S, Pawar-Patil J, Walimbe A, Patil SS, Choudhury ML, Shastri J, Agrawal S, Pawar S, Lole K, Abraham P, Cherian S; ICMR-NIV NIC Team. Phylogenetic classification of the whole-genome sequences of SARS-CoV-2 from India & evolutionary trends. Indian J Med Res. 2021 Jan & Feb;153(1 &2):166-174. doi: 10.4103/ijmr.IJMR_3418_20. Erratum in: Indian J Med Res. 2021Apr;153(4):515. PMID: 33818474; PMCID: PMC8184080.

  • 4. Bhukya PL, C VK, Lole KS. Transcriptome analysis of hepatoma cells transfected with Basal Core Promoter (BCP) and Pre-Core (PC) mutant hepatitis B virus full genome construct. J Gen Virol. 2021 Mar;102(3). doi:10.1099/jgv.0.001568. Epub 2021 Feb 17. PMID: 33595430.

  • 5. Alagarasu K, Potdar VA, Vipat V, Hundekar S, Gunjikar R, Choudhary ML, Abraham P, Lole KS. Utility of a modified heat inactivation method for direct detection of SARS-CoV-2 by RT-qPCR in viral transport medium bypassing RNA extraction: A preliminary study. Indian J Med Res. 2020 Jul & Aug;152(1 & 2):108-110. doi: 10.4103/ijmr.IJMR_3121_20. PMID: 32952145; PMCID: PMC7853279.

  • 6. Alagarasu K, Choudhary ML, Lole KS, Abraham P, Potdar V. Evaluation of RdRp & ORF-1b-nsp14-based real-time RT-PCR assays for confirmation of SARS-CoV-2 infection: An observational study. Indian J Med Res. 2020 May;151(5):483-485. doi: 10.4103/ijmr.IJMR_1256_20. PMID: 32474555; PMCID: PMC7530456.

  • 7. Deoshatwar AR, Gurav YK, Lole KS. Declining trends in Hepatitis A seroprevalence over the past two decades, 1998-2017, in Pune, Western India. Epidemiol Infect. 2020 May 8;148:e121. doi: 10.1017/S0950268820000953. PMID: 32381137; PMCID: PMC7339083.

  • 8. Pawar SD, Keng SS, Tare DS, Thormothe AL, Sapkal GN, Anukumar B, Lole KS, Mullick J, Mourya DT. A virus precipitation method for concentration & detection of avian influenza viruses from environmental water resources & its possible application in outbreak investigations. Indian J Med Res. 2019 Dec;150(6):612-619. doi: 10.4103/ijmr.IJMR_1697_18. PMID: 32048625; PMCID: PMC7038801.

  • 9. Gurav YK, Retheesh Babu G, Vinu KP, Lole KS. Suspected spread of hepatitis A virus from a restaurant among adults in rural area of the Kerala state, India. Epidemiol Infect. 2019 Jan;147:e210. doi: 10.1017/S0950268819000967. PMID: 31364560; PMCID: PMC6624875.

  • 10. Joshi MS, Lole KS, Barve US, Salve DS, Ganorkar NN, Chavan NA, Shinde MS, Gopalkrishna V. Investigation of a large waterborne acute gastroenteritis outbreak caused by group B rotavirus in Maharashtra state, India. J Med Virol. 2019 Oct;91(10):1877-1881. doi: 10.1002/jmv.25523. Epub 2019 Jul 5. PMID: 31276221; PMCID: PMC7167004.

  • 11. Arankalle VA, Srivastava N, Kushwaha KP, Sen A, Ramdasi AY, Patel PA, Kuthe S, Haldipur B, Sakpal GN, Lole KS, Ingle NB. Detection of human parvovirus 4 DNA in the patients with acute encephalitis syndrome during seasonal outbreaks of the disease in Gorakhpur, India. Emerg Microbes Infect. 2019;8(1):130-138. doi: 10.1080/22221751.2018.1563455. PMID: 30866767; PMCID: PMC6455185.

  • 12. Roy P, Patel A, Lole K, Gupta RM, Kumar A, Hazra S. Prevalence and genotyping pattern of hepatitis C virus among patients on maintenance hemodialysis at five centers in Pune, India. Med J Armed Forces India. 2019 Jan;75(1):74-80. doi: 10.1016/j.mjafi.2018.08.001. Epub 2018 Nov 22. PMID:30705482; PMCID: PMC6349655.

  • 13. Lole K, Bhukya Prudhvi Lal, and Haldipur Bangari. Hepatitis E virus In ‘Handbook of Foodborne Diseases’, Food Microbiology Series (series editor: Dongyou Liu), CRC press, Taylor & Francis Group. 2018.

  • 14. Kulkarni R, Lole K, Chitambar SD. Use of rabbit antibodies raised against Norovirus GII.4 virus-like particles for diagnosis of Norovirus infection. Indian J Med Res. 2017 Dec;146(6):788-790. doi: 10.4103/ijmr.IJMR_463_16. PMID: 29664039; PMCID: PMC5926352.

  • 15. Lole KS, Prudhvi Lal Bhukya, Subhashis Chatterjee, Hepatitis E virus, in ‘Laboratory Models for foodborne infections’, Ed. Dongyou Liu, CRC press, Taylor & Francis Group. 2017, pp 41-74.

  • 16. Haldipur B, Bhukya PL, Arankalle V, Lole K. Positive Regulation of Hepatitis E Virus Replication by MicroRNA-122. J Virol. 2018 May 14;92(11):e01999-17. doi: 10.1128/JVI.01999-17. PMID: 29540601; PMCID: PMC5952168.

  • 17. Lole K, Thorat N, Akolkar D, Pingle S. Detection of newly discovered KIs virus in India. Virus Res. 2017 Oct 15;242:146-148. doi: 10.1016/j.virusres.2017.09.021. Epub 2017 Sep 28. PMID: 28964879.

  • 18. Ingle NB, Virkar RG, Agnihotri K, Sharma KS, Lole KS, Arankalle VA. Evaluation of Liposome, Heat-Killed Mycobacterium w, and Alum Adjuvants in the Protection Offered by Different Combinations of Recombinant HA, NP proteins, and M2e Against Homologous H5N1 Virus. Viral Immunol. 2016 Oct;29(8):478-486. doi: 10.1089/vim.2016.0021. Epub 2016 Aug 10. PMID: 27508998.

  • 19. Mahilkar S, Paingankar MS, Lole KS. Hepatitis E virus RNA-dependent RNA polymerase: RNA template specificities, recruitment and synthesis. J Gen Virol. 2016 Sep;97(9):2231-2242. doi: 10.1099/jgv.0.000528. Epub 2016 Jun 20. PMID: 27324050.

  • 20. 18: Devhare PB, Desai S, Lole KS. Innate immune responses in human hepatocyte- derived cell lines alter genotype 1 hepatitis E virus replication efficiencies. Sci Rep. 2016 May 27;6:26827. doi: 10.1038/srep26827. PMID: 27230536; PMCID: PMC4882509.

  • 21. Ojha NK, Lole KS. Hepatitis E virus ORF1 encoded macro domain protein interacts with light chain subunit of human ferritin and inhibits its secretion. Mol Cell Biochem. 2016 Jun;417(1-2):75-85. doi: 10.1007/s11010-016-2715-0. Epub 2016 May 12. PMID: 27170377; PMCID: PMC7089094.

  • 22. Chatterjee SN, Devhare PB, Pingle SY, Paingankar MS, Arankalle VA, Lole KS. Hepatitis E virus (HEV)-1 harbouring HEV-4 non-structural protein (ORF1) replicates in transfected porcine kidney cells. J Gen Virol. 2016 Aug;97(8):1829-1840. doi: 10.1099/jgv.0.000478. Epub 2016 Apr 12. PMID: 27072797.

  • 23. Kulkarni R, Lole K, Chitambar SD. Seroprevalence of antibodies against GII.4 norovirus among children in Pune, India. J Med Virol. 2016 Sep;88(9):1636-40. doi: 10.1002/jmv.24495. Epub 2016 Feb 24. PMID: 26868418.

  • 24. Ojha NK, Lole KS. Hepatitis E virus ORF1 encoded non structural protein-host protein interaction network. Virus Res. 2016 Feb 2;213:195-204. doi: 10.1016/j.virusres.2015.12.007. Epub 2015 Dec 12. PMID: 26689634.

  • 25. Hundekar S, Thorat N, Gurav Y, Lole K. Viral excretion and antibody titers in children infected with hepatitis A virus from an orphanage in western India. J Clin Virol. 2015 Dec;73:27-31. doi: 10.1016/j.jcv.2015.10.012. Epub 2015 Oct 19. PMID: 26521227.

  • 26. Gurav YK, Hundekar S, Deoshatwar AR, Lole KS. Outbreak of hepatitis A in an orphanage in Pune city, India. International Journal of Tropical Medicine and Public Health, 2015; 5(2): 1-4

  • 27. Devhare P, Sharma K, Mhaindarkar V, Arankalle V, Lole K. Analysis of helicase domain mutations in the hepatitis E virus derived from patients with fulminant hepatic failure: effects on enzymatic activities and virus replication. Virus Res. 2014 May 12;184:103-10. doi: 10.1016/j.virusres.2014.02.018. Epub 2014 Mar 11. PMID: 24630891; PMCID: PMC7172619.

  • 28. Mhaindarkar V, Sharma K, Lole KS. Mutagenesis of hepatitis E virus helicase motifs: effects on enzyme activity. Virus Res. 2014 Jan 22;179:26-33. doi: 10.1016/j.virusres.2013.11.022. Epub 2013 Dec 11. PMID: 24333153.

  • 29. Devhare PB, Chatterjee SN, Arankalle VA, Lole KS. Analysis of antiviral response in human epithelial cells infected with hepatitis E virus. PLoS One. 2013 May 9;8(5):e63793. doi: 10.1371/journal.pone.0063793. PMID: 23671700; PMCID: PMC3650073.

  • 30. Chatterjee SN, Devhare PB, Lole KS. Detection of negative-sense RNA in packaged hepatitis E virions by use of an improved strand-specific reverse transcription-PCR method. J Clin Microbiol. 2012 Apr;50(4):1467-70. doi: 10.1128/JCM.06717-11. Epub 2012 Jan 11. PMID: 22205803; PMCID: PMC3318542.

  • 31. Karpe YA, Aher PP, Lole KS. NTPase and 5'-RNA triphosphatase activities of Chikungunya virus nsP2 protein. PLoS One. 2011;6(7):e22336. doi: 10.1371/journal.pone.0022336. Epub 2011 Jul 19. PMID: 21811589; PMCID: PMC3139623.

  • 32. Karpe YA, Lole KS. Deubiquitination activity associated with hepatitis E virus putative papain-like cysteine protease. J Gen Virol. 2011 Sep;92(Pt 9):2088-2092. doi: 10.1099/vir.0.033738-0. Epub 2011 Jun 8. PMID: 21653754.

  • 33. Arankalle VA, Gandhi S, Lole KS, Chadha MS, Gupte GM, Lokhande MU. An outbreak of hepatitis B with high mortality in India: association with precore, basal core promoter mutants and improperly sterilized syringes. J Viral Hepat. 2011 Apr;18(4):e20-8. doi: 10.1111/j.1365-2893.2010.01391.x. Epub 2010 Nov 26. PMID: 21108697.

  • 34. Arankalle VA, Lole KS, Arya RP, Tripathy AS, Ramdasi AY, Chadha MS, Sangle SA, Kadam DB. Role of host immune response and viral load in the differential outcome of pandemic H1N1 (2009) influenza virus infection in Indian patients. PLoS One. 2010 Oct 1;5(10):e13099. doi: 10.1371/journal.pone.0013099. PMID: 20957032; PMCID: PMC2948498.

  • 35. Karpe YA, Lole KS. RNA 5'-triphosphatase activity of the hepatitis E virus helicase domain. J Virol. 2010 Sep;84(18):9637-41. doi: 10.1128/JVI.00492-10. Epub 2010 Jun 30. PMID: 20592074; PMCID: PMC2937651.

  • 36. Karpe YA, Lole KS. NTPase and 5' to 3' RNA duplex-unwinding activities of the hepatitis E virus helicase domain. J Virol. 2010 Apr;84(7):3595-602. doi: 10.1128/JVI.02130-09. Epub 2010 Jan 13. PMID: 20071563; PMCID: PMC2838093.

  • 37. Shrivastava S, Lole KS, Tripathy AS, Shaligram US, Arankalle VA. Development of candidate combination vaccine for hepatitis E and hepatitis B: a liposome encapsulation approach. Vaccine. 2009 Nov 5;27(47):6582-8. doi: 10.1016/j.vaccine.2009.08.033. Epub 2009 Sep 9. PMID: 19747579.

  • 38. Chadha MS, Lole KS, Bora MH, Arankalle VA. Outbreaks of hepatitis A among children in western India. Trans R Soc Trop Med Hyg. 2009 Sep;103(9):911-6. doi: 10.1016/j.trstmh.2008.11.014. Epub 2009 Jan 19. PMID: 19155033.

  • 39. Arankalle VA, Lole KS, Deshmukh TM, Srivastava S, Shaligram US. Challenge studies in Rhesus monkeys immunized with candidate hepatitis E vaccines: DNA, DNA-prime-protein-boost and DNA-protein encapsulated in liposomes. Vaccine. 2009 Feb 11;27(7):1032-9. doi: 10.1016/j.vaccine.2008.11.097. Epub 2008 Dec 16. PMID: 19095027.

  • 40. Arankalle VA, Lole KS, Deshmukh TM, Chobe LP, Gandhe SS. Evaluation of human (genotype 1) and swine (genotype 4)-ORF2-based ELISAs for anti-HEV IgM and IgG detection in an endemic country and search for type 4 human HEV infections. J Viral Hepat. 2007 Jun;14(6):435-45. doi: 10.1111/j.1365-2893.2006.00801.x. PMID:17501765.

  • 41. Deshmukh TM, Lole KS, Tripathy AS, Arankalle VA. Immunogenicity of candidate hepatitis E virus DNA vaccine expressing complete and truncated ORF2 in mice. Vaccine. 2007 May 30;25(22):4350-60. doi: 10.1016/j.vaccine.2007.03.040. Epub 2007 Apr 9. PMID: 17459540.

  • 42. Chitambar S, Joshi M, Lole K, Walimbe A, Vaidya S. Cocirculation of and coinfections with hepatitis A virus subgenotypes IIIA and IB in patients from Pune, western India. Hepatol Res. 2007 Feb;37(2):85-93. doi: 10.1111/j.1872-034X.2007.00025.x. PMID: 17300702.

  • 43. Arankalle VA, Sarada Devi KL, Lole KS, Shenoy KT, Verma V, Haneephabi M. Molecular characterization of hepatitis A virus from a large outbreak from Kerala, India. Indian J Med Res. 2006 Jun;123(6):760-9. PMID: 16885597.

  • 44. Capoulade-Métay C, Ayouba A, Kfutwah A, Lole K, Pêtres S, Dudoit Y, Deterre P, Menu E, Barré-Sinoussi F, Debré P, Theodorou I. A natural CCL5/RANTES variant antagonist for CCR1 and CCR3. Immunogenetics. 2006 Jul;58(7):533-41. doi: 10.1007/s00251-006-0133-2. Epub 2006 Jun 22. PMID: 16791620.

  • 45. Lole KS, Arankalle VA. Quantitation of hepatitis B virus DNA by real-time PCR using internal amplification control and dual TaqMan MGB probes. J Virol Methods. 2006 Jul;135(1):83-90. doi: 10.1016/j.jviromet.2006.02.004. Epub 2006 Mar 23. PMID: 16551481.

  • 46. Chobe LP, Lole KS, Arankalle VA. Full genome sequence and analysis of Indian swine hepatitis E virus isolate of genotype 4. Vet Microbiol. 2006 May 31;114(3-4):240-51. doi: 10.1016/j.vetmic.2005.12.007. Epub 2006 Feb 2. PMID: 16457969.

  • 47. Lole KS, Jha JA, Shrotri SP, Tandon BN, Prasad VG, Arankalle VA. Comparison of hepatitis C virus genotyping by 5' noncoding region- and core-based reverse transcriptase PCR assay with sequencing and use of the assay for determining subtype distribution in India. J Clin Microbiol. 2003 Nov;41(11):5240-4. doi: 10.1128/JCM.41.11.5240-5244.2003. PMID: 14605173; PMCID: PMC262521.

  • 48. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS, Novak NG, Ingersoll R, Sheppard HW, Ray SC. Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol. 1999 Jan;73(1):152-60. doi: 10.1128/JVI.73.1.152-160.1999. PMID: 9847317; PMCID: PMC103818.

  • 49. Patil NS, Lole KS, Deobagkar DN. Adaptive larval thermotolerance and induced cross-tolerance to propoxur insecticide in mosquitoes Anopheles stephensi and Aedes aegypti. Med Vet Entomol. 1996 Jul;10(3):277-82. doi: 10.1111/j.1365-2915.1996.tb00743.x. PMID: 8887340.

  • 50. Samant SA, Vilekar NR, Lole KS, Deobagkar DD, Deobagkar DN. Characterization of MboI repeat DNA sequence of Anopheles stephensi. Indian J Biochem Biophys. 1991 Oct-Dec;28(5-6):499-503. PMID: 1812089.

 

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